<i>In-silico</i> Rational Protein Engineering and Design Approach to Improve Thermostability of a Haloalkane Dehalogenase Enzyme
نویسندگان
چکیده
منابع مشابه
computer aided design of a luciferase like haloalkane dehalogenase enzyme by homology-based rational protein design (hrpd) method
in this report, we describe a procedure for in-silico design of a novel haloalkane dehalogenase protein that exhibits luciferase property which can be potentially used in biosensor applications. from a pdb blast search, the selected haloalkane dehalogenase (pdb code: 1ede) had a close structural homology with a lucifearse (pdb code: 2psj chain a) sharing an identity of 33%. initially, the amino...
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15 صفحه اولSpecificity and kinetics of haloalkane dehalogenase.
Haloalkane dehalogenase converts halogenated alkanes to their corresponding alcohols. The active site is buried inside the protein and lined with hydrophobic residues. The reaction proceeds via a covalent substrate-enzyme complex. This paper describes a steady-state and pre-steady-state kinetic analysis of the conversion of a number of substrates of the dehalogenase. The kinetic mechanism for t...
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ژورنال
عنوان ژورنال: American Journal of Bioinformatics
سال: 2015
ISSN: 1948-9862
DOI: 10.3844/ajbsp.2015.34.46